MICROSATELLITE DNA MARKER FOR MOLECULAR CHARACTERIZATION OF AFRICAN MAIZE (Zea mays L.) LANDRACES

Maize (Zea mays L.).

Agrobacterium tumefaciens-mediated transformation is an effective method for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an Agrobacterium strain carrying a standard binary vector. In addition to step-by-step laboratory transformation proced...

Patterns of DNA sequence polymorphism along chromosome 1 of maize (Zea mays ssp. mays L.).

We measured sequence diversity in 21 loci distributed along chromosome 1 of maize (Zea mays ssp. mays L.). For each locus, we sequenced a common sample of 25 individuals representing 16 exotic landraces and nine U.S. inbred lines. The data indicated that maize has an average of one single nucleotide polymorphism (SNP) every 104 bp between two randomly sampled sequences, a level of diversity hig...

Inositol 1,3,4,5,6-pentakisphosphate 2-kinase from maize (Zea mays L.): molecular and biochemical characterization

Association Mapping for Enhancing Maize (Zea mays L.) Genetic Improvement

Association mapping through linkage disequilibrium (LD) analysis is a powerful tool for the dissection of complex agronomic traits and for the identifi cation of alleles that can contribute to the enhancement of a target trait. With the developments of high throughput genotyping techniques and advanced statistical approaches as well as the assembling and characterization of multiple association...

A strategy for assembling the maize (Zea mays L.) genome

UNLABELLED Because the bulk of the maize (Zea mays L.) genome consists of repetitive sequences, sequencing efforts are being targeted to its 'gene-rich' fraction. Traditional assembly programs are inadequate for this approach because they are optimized for a uniform sampling of the genome and inherently lack the ability to differentiate highly similar paralogs. RESULTS We report the developme...